Find reconstructions that secrete all of one set of metabolites and take up all of another — e.g. “secretes butyrate and takes up lactate”, a cross-feeding-candidate filter. Predicted capability (max potential, unlimited medium; APOLLO + AGORA2). Add up to 5 metabolites in total.
Add at least one metabolite above.
Source: Supplementary Table S3 of Heinken A, Hulshof TO, Nap B, … Thiele I. A genome-scale metabolic reconstruction resource of 247,092 diverse human microbes… Cell Systems 2025;16(2):101196. PMID: 39947184.